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Optimization of biocatalytic synthesis of Chitosan Ester using response surface methodology | ||
| Chemistry of Solid Materials | ||
| مقاله 5، دوره 1، شماره 2، تیر 2013، صفحه 41-55 اصل مقاله (3.65 M) | ||
| نویسندگان | ||
| Naz Chaibakhsh؛ M.B. Abdul Rahman* ؛ K. Jesunathan؛ M Basri | ||
| چکیده | ||
| Esterification of chitosan with adipic acid catalyzed by immobilized Candida antarctica lipase B was carried out in this study. Response surface methodology (RSM) based on a four-factor- five-level small central composite design (SCCD) was employed to model and analyze the reaction. A total of 21 experiments representing different combinations of the four reaction parameters including chitosan concentration (0.01-0.10 g/mL), temperature (35-65◦C), time (15-240 min), and enzyme amount (5-20 % w/w of total substrate) were generated. A partial cubic equation was fitted to the data with a R2 of 0.9738 showing a high correlation between predicted and experimental values. The enzyme was capable of esterifying chitosan with a maximum yield of 45.3%. Chitosan concentration was found to be the most significant parameter which influenced the ester synthesis. At the optimal conditions of a short reaction time 15 min, 65oC, 0.10 g/mL of chitosan and 18.7% of enzyme, the esterification percentage was 43.5%. | ||
| کلیدواژهها | ||
| Chitosan; Enzymatic synthesis; Esterification; modeling; Response surface methodology | ||
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