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Biological mechanisms for the effects of lipopolisacharide Escherichia coli on the central nervous system cells | ||
| Caspian Journal of Environmental Sciences | ||
| دوره 24، شماره 4، دی 2026، صفحه 1139-1146 اصل مقاله (1.05 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22124/cjes.2026.10028 | ||
| نویسندگان | ||
| Ayagoz Mendigaliyeva1؛ Roza Bakessova* 1؛ Assel Arystanova* 1؛ Alisher Bekmuratov2؛ Anara Dzhaldybaeva3؛ Rimma Tashmetova4؛ Zaure Ibragimova5؛ Georgiy Afonin6 | ||
| 1Department of Natural Science Disciplines, Institute of Education and Management, West Kazakhstan Innovative Technological University, Uralsk, Kazakhstan | ||
| 2Research Scientist, “NAUCHNYI HOLDING” LLP, Almaty, Kazakhstan | ||
| 3Department of Biology, Faculty of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Almaty, Kazakhstan | ||
| 4Municipal State Institution “Center for Support of Children in Need of Special Social Services No. 1” of the Education Department of Almaty, Kazakhstan | ||
| 5Department of Pharmaceutical and Toxicological Chemistry, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan | ||
| 6Department of Oncology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan | ||
| چکیده | ||
| The main activator of the inflammation response Escherichia coli lipopolysaccharide (LPS) demonstrates an enormous influence on cells of the central nervous system. The aim of the current investigation is to understand the molecular and cellular mechanisms of action of LPS on neurons, microglia, and astrocytes. Experimental design was in vitro. Isolation of cells from brain tissues of Wistar rats and their cultivation was carried out in three groups including control, low-concentration LPS treatment (100 ng mL-1), and high-concentration LPS treatment (1000 ng mL-1). All manipulations lasted for 24 hours and were repeated in triplicates. The viability of cells was estimated by means of the MTT assay, activity of antioxidant enzymes – by spectrophotometrically, and the inflammatory genes expression was estimated with the help of quantitative RT-PCR (qRT-PCR). Inflammation proteins’ concentration was estimated with ELISA. Analysis of results was carried out with One-Way ANOVA with post-hoc Tukey’s test. It has been shown that viability of neurons has reduced from 98.4% to 61.3% at the high concentration. The lowest sensitivity have demonstrated microglia with viability reduction from 97.8% to 72.5%. The activity of superoxide dismutase reduced from 42.6 to 24.8 U mg-1 protein, whereas the level of malondialdehyde increased from 1.24 to 4.92 nmol mg-1 protein. There was a 8.76 fold increase in the gene expression of TNF-α and its level also increased from 12.4 to 82.4 pg mL-1. From the correlation matrix, the most correlated (with a coefficient of 0.88) parameters were TNF-α and IL-1β. Malondialdehyde showed a correlation of 0.84 with TNF-α, whereas superoxide dismutase showed a correlation of -0.68 with TNF-α. All of these coefficients were significant at the 0.01 level. From the correlation network, the central node was found to be malondialdehyde and the protective node superoxide dismutase. According to the results, LPS, when it activates TLR4, sets off a chain reaction that involves inflammation and oxidative responses leading to neuronal damage. Targeting both pathways at the same time is an efficient approach in future treatments. This framework can be used to understand the mechanisms involved in neuroinflammatory diseases and to find new therapeutic targets. | ||
| کلیدواژه‌ها | ||
| Lipopolysaccharide؛ Neuroinflammation؛ Oxidative stress؛ Central nervous system | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 8 تعداد دریافت فایل اصل مقاله: 9 |
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